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Fig. 3. Simultaneous imaging (co-localization) of mitochondrial flavoproteins (A)
and calcium (B) in permeabilized myocardial fibers loaded with Rhod-2. (A) As
in the case of isolated cells, autofluorescence of mitochondrial flavoproteins
in a fully oxidized state in the absence of mitochondrial substrates shows a
regular arrangement of mitochondria in saponin-permeabilized fibers. (B)
Fluorescence of Rhod-2 trapped in mitochondria allows the localization and
quantification of mitochondrial free calcium. As in cardiomyocytes
(Fig. 1), clear co-localization
of flavoproteins (green fluorescence) and mitochondrial calcium (red
fluorescence) can be seen.
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