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Fig. 1. Simultaneous imaging (co-localization) of mitochondrial flavoproteins (A)
and calcium (B) in permeabilized cardiomyocytes loaded with Rhod-2. (A)
Autofluorescence of mitochondrial flavoproteins in a fully oxidized state in
the absence of mitochondrial substrates shows a regular arrangement of
mitochondria in saponin-permeabilized cells. (B) Fluorescence of Rhod-2
trapped in mitochondria allows the localization and quantification of
mitochondrial free calcium. Clear co-localization of flavoproteins (green
fluorescence) and mitochondrial calcium (red fluorescence) can be seen.
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