spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Localisation of vasoactive intestinal polypeptide (VIP)-binding sites to the chromaffin cell subtypes in the posterior cardinal vein of rainbow trout using triple labelling. (A) Dopamine ß-hydroxylase (DßH)-immunoreactive cells (mouse anti-DßH). (B) Phenylethanolamine N-methyl transferase (PNMT)-immunoreactive cells (rabbit anti-bovine PNMT). (C) Labelling of VIP-binding sites. (D) Overlay image of the sections shown in A and B (DßH- and PNMT-positive cells); the PNMT-negative cells are indicated by arrows. (E) Overlay image of the sections shown in A and C (DßH and VIP labelling). (F) Overlay image of the sections shown in B and C (PNMT and VIP labelling); the PNMT-negative cells are indicated by arrows. Yellow/orange colour indicates co-localisation. Note that only a few of the DßH-labelled cells were negative for PNMT, thus indicating only a small number of noradrenaline-containing cells. Moreover, the pattern of VIP binding was identical to that of DßH, indicating that all chromaffin cells possess VIP-binding sites. Scale bars A–F, 150 µm.





Right arrow Return to article