Fig. 6. Immunocytochemical staining of vacuolar ATPase B subunit isoforms vatB1 and vatB2 in the small intestine of the zebrafish. (A) No background staining was observed in the negative control without primary antibody, except in the region of the microvilli, which contains levamisol-resistant alkaline phosphatase. (B) Antibody #1035, which is specific for the vatB1 isoform, resulted in a weak staining of cells. The microvilli showed unspecific staining, and some cells in the central region of the villi near the blood vessels of the lamina propria showed a positive reaction. (C) Antibody #1034, which binds to vatB1 and vatB2, resulted in a very strong signal in large cells at the base of the villi, which according to their position and shape most likely represent neurosecretory cells. mc, mucus cells; lp, lamina propria. Arrows identify large positive cells. Scale bars, 50 µm.

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