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Fig. 5. Immunocytochemical staining of vacuolar ATPase B subunit isoforms vatB1 and vatB2 in zebrafish liver. (A) No background staining was observed in the negative control without primary antibody. (B) Antibody #1035, which is specific for the vatB1 isoform, resulted in a weak staining of hepatocytes. (C) Antibody #1034, which binds to vatB1 and vatB2, resulted in a very strong signal in most hepatocytes. Scale bars, 50 µm.





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