Fig. 1. Accumulation of myofibrillar proteins in the wild-type and thick filament null mutants. The accumulation of contractile proteins in myofibrillar preparations from animals continuously labeled for 3 days with low specific activity ³⁵S-labeled bacteria was quantified following autoradiography of 8% SDS–PAGE gels. (A) the autoradiogram of the wild-type N2 (lane a), the myosin B null CB190 (lane b) and the paramyosin null CB1214 (lane c). (B) Graph of the relative rates of accumulation of myosin and paramyosin normalized to actin reveals that paramyosin accumulation is reduced by 33% in the myosin null, and myosin accumulation is reduced by 28% in the paramyosin null. Quantification is based on four independent determinations on each strain, and values are expressed as means ± S.D.

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