Fig. 4. PDH-immunoreactivity in an accessory medulla (AMe)-explant as used for transplantations (A) and in the central brains of two postoperatively rhythmic cockroaches (B–D). (A) In the 10-µm paraffin section of an excised AMe-graft, two large- and two medium-sized PDH-ir medulla neurons (PDH-Me) send processes into the AMe. Counterstaining with methylene blue shows unstained somata next to the PDH-Me. (B) Reconstruction of PDH-immunoreactivity in the brain of a postoperatively rhythmic cockroach (animal ID 11/16; Tables 2, 3). Three large- and two medium-sized grafted PDH-ir cells in the antennal lobe (AL; arrow) project via new routes to original arborisation sites in the superior medial and superior lateral protocerebra (SMP and SLP, respectively). Faintly stained PDH-ir neurons in the pars intercerebralis (PI) give rise to spotted staining in the protocerebrum, which can be clearly distinguished from regenerated fibres. a, alpha lobe. (C) Frontal brain section of the animal (animal ID 13/84; Tables 2, 3) in Fig. 2 with regenerated PDH-ir arborisations in the SMP and SLP (arrowheads) and antennal lobe (AL, open arrowhead). Inset: grafted large PDH-ir soma in the anterior AL (arrow). Ca, calyces of the mushroom bodies. (D) A more posterior slice of the same brain shows regenerated fibres invading the protocerebrum via the antenno-glomerular tract (arrowheads). Scale bars: 50 µm (A), 200 µm (B), 100 µm (C,D).

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