Fig. 1. Plasmid maps and predicted structure of dsRNA transcripts. (A) Schematic representation of target plasmid pMinEGFP and control plasmid pMiRED. (B) Design of EGFP inverted repeats (EGFP-IR) and predicted structure of dsRNA transcript. Filled arrows pointing in opposite directions represent the EGFP-IR. Spacer regions are shown. (C) Schematic representation of generic dsRNA-expression plasmid, pIR-EGFP. Each IR-EGFP construct was cloned into pMiRED within the arms of minos. Actin5C, D. melanogaster actin5C promoter; HspT, D. melanogaster Hsp70 terminator sequence. The EGFP-, EGFP-IR- and DsRed-encoding genes are indicated by filled arrows. ML, minos left arm; MR, minos right arm.

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