Fig. 9. The effects of forskolin and 1,9-dideoxyforskolin on odorant-evoked responses. (A) Representative electro-olfactogram (EOG) recordings to a mixture of 100 µmol l^–1 L-amino acids (AA; alanine, arginine, glutamate and methionine), polyamines (PA; 20 µmol l^–1 putrescine, cadaverine and 2 µmol l^–1 spermine) and 10 µmol l^–1 bile salts (BS; sodium taurocholate and taurolithocholate) prior to (i), during (ii) and after (iii) adaptation to 7 µmol l^–1 forskolin (For). DS, dimethyl sulfoxide; C, CFTW control). (B) Representative EOG recordings to 100 µmol l^–1 AA, 1 µmol l^–1 BS and PA (10 µmol l^–1 putrescine, cadaverine and 1 µmol l^–1 spermine) during adaptation to 20 µmol l^–1 1,9-dideoxyforskolin (De). (C) Percentage of unadapted response (mean ± S.D.) to mixtures of L-amino acids (50–500 µmol l^–1), polyamines (1–20 µmol l^–1), bile salts (10–50 µmol l^–1), forskolin control and ATP (30–40 µmol l^–1) during adaptation to forskolin (5–20 µmol l^–1). Numbers associated with each bar indicate the number of fish tested for each odorant. The adapting solution is underlined. X, Y and Z designate statistical significance across groups (one-way ANOVA; Tukey's post hoc test, P<0.05).

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