Fig. 3. The differential effect of a transcription and a translation blocker on ITM formation in Lymnaea. (A) A naïve group of snails (N=20) was injected with the transcriptional protein synthesis inhibitor Actinomycin D 2.5 h before the training session. Following the 0.5 h training session (1) the snails were tested for memory 3 h later (MT). Memory was present. That is, the number of attempted pneumostome openings in the memory test session was significantly less than the number of attempted openings in the operant conditioning training session (P<0.01). To show that this was a case of memory and not a drug-induced side effect, we altered the context 1 h later. When challenged with the `carrot context' (see text) the snails attempted as many pneumostome openings as they did in the training session (P>0.05) and statistically more than in the memory test session (P<0.01). (B) As in A, except that snails were injected with the translational protein synthesis blocker Anisomycin. No ITM was observed when tested 3 h after the training session. That is, the number of attempted pneumostome openings in the memory test session was not statistically different (NSD) from the number in the training session (P>0.05).

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