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Fig. 2. (A) Buffer-corrected far-ultraviolet circular dichroism (CD) spectra of Gillichthys mirabilis and G. seta muscle-type lactate dehydrogenases (A4-LDHs) at 20 and 40 °C. (B) Thermal denaturation profiles of the A4-LDH forms monitored using CD spectroscopy; absorbance was measured at 222 nm. Curves were fitted as described in the text; G. mirabilis A4-LDH Tm=60.2±0.1 °C and G. seta A4-LDH Tm=58.4±0.1 °C). {Theta}MRW, residue ellipticity.





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