Fig. 4. The cellular location of punctate kfCFTR immunofluorescence in FW controls
(a small minority of cells) and SW animals (all cells), as determined by
optical sections in the confocal microscope, was in apical crypts
approximately 7 µm deep. The transitional phases included many cells with
punctate kfCFTR immunofluorescence well below the surface. It would appear
kfCFTR distribution in FW is diffuse, then condenses in the first 24 h after
salinity transfer, prior to moving kfCFTR to the apical membrane at
approximately 48 h. Columns with different letters are significantly different
(P<0.05 or better; single-classification ANOVA followed by a
Bonferroni post-test).
