Fig. 8. Endocytosis of the fluorescent endocytic marker FM 4-64 to vacuolar
membranes is slowed down in V-ATPase null mutants. (A) Wild-type (WT) and
vma3
cells were stained with FM 4-64 as described in Materials
and methods. Samples were removed for microscopy after staining for the
indicated times (min), in order to monitor the internalization kinetics of the
dye. (B) The indicated strains were stained with FM 4-64 and photographed
after a 60 min chase in YPD medium. Top panels show FM 4-64 fluorescence;
bottom panels show visible light images of the same cells.
