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Fig. 6. stv1{Delta} and vph1{Delta} strains show defects in vacuolar acidification. Wild-type (WT) and V-ATPase mutants were grown in YPD medium, pH 5.5, and stained with LysoSensor Green DND-189 as described in Materials and methods. Staining of vph1{Delta} and stv1{Delta}/vph1{Delta} strains is shown at two different light intensities (*3 is triple intensity). The panel below shows staining of vph1{Delta} grown in YPD buffered at pH 7.5.





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