Fig. 3. NOS and VIP immunoreactivities and NADPH-diaphorase reactivity in the
gastrointestinal tract of Xenopus laevis. (A,B) Double-staining of
the myenteric plexus (mep) of the proximal intestine showing NOS- (A) and
VIP-immunoreactive nerve fibres (B) but only NOS-immunoreactive nerve cell
bodies. Most VIP-immunoreactive nerve fibres are not NOS-immunoreactive. (C)
NOS-immunoreactive endocrine cell in the mucosa of the proximal intestine.
(D,E) Section through the proximal intestine showing colocalisation between
NOS- (D) and VIP-immunoreactive nerve fibres (E) in the circular muscle layer
(cm). (F,G) Double-staining of the myenteric plexus of the distal intestine
showing NOS- (F) and VIP-immunoreactive nerve fibres (G) but only
NOS-immunoreactive nerve cells bodies. Most VIP-immunoreactive nerve fibres
are not NOS-immunoreactive. (H) NOS-immunoreactive nerve cell body in the
myenteric plexus of the rectum. (I) NADPH-diaphorasereactive nerve cell body
and nerve fibres in the myenteric plexus of the rectum. Bars: 25 µm
(D,E,H), 50 µm (A—C,F,G,I).
