
Fig. 2. PACAP, VIP and helospectin immunoreactivities in the gastrointestinal tract
of Xenopus laevis. (A,B) Double-staining of a section through the
cardiac stomach showing a high degree of colocalisation between PACAP (A) and
VIP (B) immunoreactivities in the myenteric plexus (mep), the circular muscle
layer (cm) and submucosa (sm). (C,D) A high degree of colocalisation between
PACAP (C) and VIP (D) immunoreactivities in nerve fibres in the myenteric
plexus of the pyloric stomach. (E,F) Colocalisation between PACAP (E) and VIP
(F) immunoreactivities in varicose nerve fibres in the circular muscle layer
of the proximal intestine. (G,H) Colocalisation between helospectin (G) and
VIP (H) immunoreactivities in varicose nerve fibres along the circular muscle
fibres in the cardiac stomach. Bars: 25 µm (E-H), 50 µm (A-D).