Fig. 5. Overall promoter structures of the Manduca sexta V-ATPase genes
mvB, mvG and mvd. Hybridization screening of a genomic
-Fix II library from M. sexta led to the isolation of three
genes encoding V-ATPase subunits B, G and d. The corresponding 5'
upstream regions were subcloned and sequenced. Fragments of 1 kb are shown,
with special emphasis on overall promoter structures in the region close to
the translational start site (shaded blue). GC content was determined using a
window size of 30 bp; GC contents of more than 50 % are in red, of between 20
% and 50 % are in grey and of less than 20 % are in blue. Black arrows,
canonical TATA boxes; red arrows, cAMP-responsive, elements (CREs); blue
arrows, ecdysterone response elements (EcREs).
