Fig. 3. Effect of 20-hydroxyecdysone and juvenile hormone III on transcript levels
of Manduca sexta V-ATPase genes mvB, mvG and mvd.
20-Hydroxyecdysone (20-HE) (200µg; approximately 0.4 mg ml-1
haemolymph) or juvenile hormone III (JHIII) (20µg; approximately 40µg
ml-1 haemolymph) was injected into the dorsal vessel of
fifth-instar larvae (days 2-3, 2 g body mass). After the indicated period of
rearing in the presence of food, total RNA was extracted from the midgut.
Pooled total RNA (2 µg) was dotted onto nylon membranes and hybridized with
digoxigenin-labelled ssRNA probes. Chemiluminescence signals were quantified
densitometrically. Values (± S.E.M., N=3) are given as a
percentage of signal intensities obtained from untreated animals and are
normalized to the transcript levels of ribosomal protein S7
(Jiang et al., 1996).
