
Fig. 9. Distribution of entrained adult eclosion (A) and responses to
CO2 during adult eclosion (BE). (A) The entrainment period
consisted of 2 weeks in a 17 h:7 h light:dark cycle coupled to a thermoperiod
of 28 °C:25 °C. The start of the scotophase is depicted by the shaded
column at 15:00 h Pacific Standard Time (PST). The frequency of eclosion is
depicted for 189 animals. (BE) The ability of CO2 to induce
premature eclosion in entrained pharate adults. Animals were treated with
CO2 for 1 min at various times (pink arrows) before lights out.
Each pair of points (open and filled circle) and a cross represent eclosion
behaviours from one animal (sample). Since the onset of the abdominal and
thoracic components of ecdysis (pink open and filled circles, respectively)
often occurred separately in CO2-treated animals, they are
separated temporally on the graph. When behaviours occurred in immediate
succession, the dots were overlaid as a circle with a dotted center. Controls,
in which abdominal and thoracic behaviours always occurred in immediate
succession (thus open circles containing black dots), are shown in black. The
time when the wings are fully expanded and move to the `moth' position is
depicted by a cross for each animal.