Fig. 6. The timing of the onset of ecdysis-triggering hormone (ETH)-induced ecdysis
in larvae injected with the cGMP-specific phosphodiesterase inhibitor
Zaprinast. (A) Animals were injected with Zaprinast 10 min prior to injection
with 200 pmol of ETH (at 0 min), at the amounts listed on the y-axis.
Controls (C) were injected with the Zaprinast solubilizing agent,
100% dimethylsulfoxide (DMSO), before injection of ETH. The timing of ecdysis
was not significantly different in these controls from that in untreated
controls (data not shown). The bars represent mean times + S.E.M. Letters
represent significant differences from the control and from each other, as
determined by analysis of variance (P<0.001) using the Tukey test
for differences (
=0.05). (B) Representative micrographs of cGMP
immunoreactivity in abdominal ganglion 3 in DMSO- (C) and
Zaprinast-treated (Z) animals 60 min after injection with ETH. Scale
bar, 100 µm.
