Fig. 2. Photographs of single-labeled (A) and double-labeled (B-D) preparations of
ganglion A3 from ecdysis-triggering hormone (ETH)-injected larvae dissected at
ecdysis. Arrows in all panels point to the L2,5 cells. (A) Video
montage of 2-3 optical sections of a cGMP-stained preparation, as visualized
with a color reaction. Axons of the L2,5 cells can be seen exiting
the ventral nerve (black arrowhead). (B-D) Collapsed Z-series confocal images
of double-labeled preparations. Red is FMRFamide (FMRFa), leucokinin (LK) or
Manduca sexta diuretic hormone (DH) staining in panels B-D,
respectively. cGMP is indicated by green (B-D) and is marked by white arrows.
Co-localization was not noted in cells L2,5 in any panel. Scale
bars, 100 µm.
