Fig. 2. HPLC chromatograms (A-D) and absorption spectra (E,F) indicating
chromophore type for six anoles. Vitamin A1 (A) and vitamin
A2 (B) standard chromatograms. HPLC chromatograms of whole eye
extracts from Anolis cristatellus (C) and A. carolinensis
(D). The peaks corresponding to the 11-cis isomers are indicated by
stars (filled star, vitamin A1 11-cis-retinaloxime; open
star, vitamin A2 11-cis-retinaloxime). The elution time of
the vitamin A1 11-cis-retinaloxime is 4.58 min while that
of the vitamin A2 11-cis-retinaloxime is 5.16 min. (E)
Absorption spectra of the 11-cis isomers from whole-eye extracts of
A. cristatellus and four other Puerto Rican anoles (trace 1, A.
gundlachi; trace 2, A. cristatellus; trace 3, A.
evermanni; trace 4, A. pulchellus; trace 5, A. krugi).
The bold trace is the spectrum of the vitamin A1
11-cis-retinaloxime standard. (F) Absorption spectrum of the
11-cis isomer from whole-eye extracts of A. carolinensis
(trace 6) and the vitamin A2 11-cis-retinaloxime standard
(bold trace). Vertical lines are drawn through the 
max of
the spectra in E and F.
