Fig. 6. Novel, developmentally regulated expression of MsNSF. Pharate adult brains
were analyzed by staining with antibodies against MsNSF (A,C,F), diuretic
hormone (DH) (B,C), eclosion hormone (EH) (D,E) or bombyxin (A). Bombyxin is
synthesized in four pairs of cells in the anterior protocerebrum (A, green)
that do not colocalize with enriched MsNSF immunoreactivity (A, red). Diuretic
hormone (DH) in later stages is localized to 80-100 neurosecretory cells (B),
which lie medial to the eNSF-IR cells, as revealed by costaining with DH and
MsNSF antibodies (C, arrows). EH in larvae is synthesized by paired
ventro-medial cells (D). In later stages of development, EH-containing
ventromedial cells in the brain migrate anteriorly, to the proctocerebrum, and
are positioned ventrally to the eNSF-IR cells (E). Axons of eNSF-IR run
parallel to EH axons to end in the CC—CA complex (F). The posterior
aspect of brains stained for EH (E) or MsNSF is shown (F). Note the axons of
eNSF-IR from Type IIa4 cells run ventrally from the dorsally
situated cell bodies (outside the plane of focus, arrow) in F; asterisks
denote Type I staining.
