Fig. 5. Expression of MsNSF is developmentally regulated in a subset of
neuroendocrine cells and is selectively enriched in specific subsites of
hormonal release in M. sexta brain. Enriched MsNSF immunoreactivity
(eNSF-IR) is present on or after day 3 of pupation and continues throughout
adult development (B-E). FMRFamide staining detects most cells of the
neurosecretory complex and various varicosities in the brain (F). (G) Paraffin
section showing exaggerated Type I staining and eNSF-IR (arrow). The image was
overexposed to show both eNSF-IR and Type I staining. eNSF-IR is present only
in the corpora cardiaca (CC) (H,I) and excluded from the corpora allata (CA).
(I) A higher magnification of eNSF-IR in varicosities of CC in neurohemal
complex. Note that no other neurosecretory cells are stained in paraffin
sections (G), ruling out problems of antibody penetration in our whole mounts.
A representative staining with pre-immune IgG is shown in A, for comparison of
specific signals with background staining.
