Fig. 1. In vivo expression of MsNSF rescues the temperature-sensitive
growth defect in sec18-1 yeast mutant. (A) Wild-type (RSY 248) or strains
derived from sec18-1 (SEY 5186)
(Table 1) containing the
`empty' vector, pYES2 (ASHY1896-1), wild-type SEC18 gene (ASHY1896-5)
or SEC18 (ASHY1896-7) and MsNSF (ASHY1896-2) cloned downstream from
the GAL1 promoter (PGAL) were grown at 24 °C. Serial
dilutions (indicated above panels) were spotted on to rich agar medium
containing 3 % galactose and incubated at 24 °C (left), 30 °C (middle)
or at 37 °C (right) for 3 days. (B) Cells were incubated at 32 °C in
rich agar medium containing 3 % galactose (left), 2 % raffinose (middle) or 5
% glucose (right) as carbon source. (C) Wild-type (X2180-1B) or cells derived
from sec18-1 containing various constructs were incubated at 37
°C in a humid chamber for 8 days and returned to 22 °C for 4 days.
