Fig. 1. Purification of toxin Cn11. Sub-fraction II-5 (1 mg), obtained by previous separation of soluble venom of the scorpion Centruroides noxius, was applied to an HPLC C4 reverse-phase column (Vydac, Hisperia, CA, USA) and eluted with a linear gradient of solvent A (0.12 % trifluoroacetic acid in water) to 60 % solvent B (0.1 % trifluoacetic acid in acetonitrile) over 60 min. Component 4 (140 µg) was further separated by HPLC using a C18 reverse-phase column (Vydac) by application of 25 % solvent B (inset). The asterisk indicates the elution position of the pure toxin.
