Fig. 7. The effects of prior heat shock, anoxia and exercise on the bioenergetic status of locust flight muscle as measured by the ratio [ArgP]/([ArgP]+[Arg]). (A) Heat shock alone did not disrupt [ArgP]/([ArgP]+[Arg]) (t-test, t=–2.05, P=0.09, d.f.=6). [ArgP]/([ArgP]+[Arg]) ratios were perturbed after 1 h under anoxic conditions in control (t-test, t=2.79, P=0.03, d.f.=6), control + recovery and heat-shock animals (three-way ANOVA with Tukey multiple pairwise comparison, F=17.22, P<0.001, d.f.=4). [ArgP]/([ArgP]+[Arg]) ratios indicated that energy reserves in all groups were restored after 2 h under anoxia. [ArgP]/([ArgP]+[Arg]) ratios increased after 1 h under anoxia despite animals being under constant anoxic conditions. The line through the control value at 0 h indicates the basal level of [ArgP]/([ArgP]+[Arg]). (B) I: heat shock alone did not significantly affect [ArgP]/([ArgP]+[Arg]). II: there was no significant difference between control animals kept at room temperature for 4 h and heat-shocked animals that had a recovery period of 1 h at room temperature. III: exercise resulted in a significant reduction in the [ArgP]/([ArgP]+[Arg]) ratio in the flight muscle of animals that had been previously subjected to heat shock. Values are means + S.E.M. (N=7–10). Asterisks indicate a significant difference between control and heat-shocked animals (P<0.05). A horizontal bar indicates no significant difference.
