Fig. 5. Ovarian uptake of hydrocarbons (HCs) in vitro. (A) [3H]3,11-Dimethylnonacosane (21.46 kBq) was topically applied onto sternites 3 and 4 of a day 5 female, and the sternites were then co-incubated in TC199 with ovaries of different ages in the presence of 1 mg ml–1 purified high-density lipophorin (HDLp). [3H]3,11-Dimethylnonacosane levels in the ovary were assayed by liquid scintillation spectrometry. (B) Sternites 3 and 4 from a day 6 female were co-incubated with an ovary from a day 6, 7, 8 or 9 female in 500 µl of TC199 medium containing 10 % hemolymph and [1-14C]propionate (25.9 kBq). Both sets of incubations were at 27°C for 24 h. Values are means ±1 S.E.M. (N=8). Some error bars are obscured by the symbol.
