Fig. 8. Western blot analysis of cystic fibrosis transmembrane conductance regulator (CFTR) in intestine and gill tissue from seawater-adapted killifish (20 µg total protein per lane). Proteins were separated on a 7 % polyacrylamide gel, transferred onto an Immobilon-P membrane and probed with anti-human CFTR monoclonal antibody (1 µg ml–1). Proteins were visualized following incubation in BCIP/NBT Blue substrate development solution. Lane A, heart tissue (negative control); lane B, gill tissue; lane C, posterior intestine. In gill and intestine, the CFTR antibody cross-reacts with a 175 kDa protein and two lighter bands at 90.3 and 94.2 kDa (N=3). Molecular mass markers (kDa) (on left): 205, 116, 97.4, 84, 66, 55, 45 and 33 (from top to bottom).
