Fig. 3. Apoptotic somatic cells in ovarian connective tissues. Ovarian sections were made at 23 (A,B), 25 (C,D), 40 (E,F) and 70 (G,H) days post-hatching. Apoptotic cells were detected by TUNEL staining (B,D,F,H) and were counterstained with haematoxylin and eosin (A,C,E,G). Large numbers of TUNEL-positive somatic cells (black and white arrowheads) were detected in the ovarian connective tissue of genetic all-females from 23 to 50 days post-hatching. ca, cortical alveolar oocyte; oc, ovarian cavity; og, oogonia; po, perinucleolar oocyte; sc, somatic cell. Scale bars, 20 µm.
