
Fig. 7. Hanssons histochemistry of whole, dissected midgut from early fourth- instar larvae of Aedes aegypti viewed at low (A) and high (B,C) magnification. Intense dark staining is observed in the gastric caeca (GC) and the posterior midgut (PM), indicating carbonic anhydrase activity. Large, relatively unstained cells are also evident in the posterior midgut (A,C). The posterior-most extensions of the lobes of the gastric caeca exhibit relatively low levels of reaction product, indicating lower levels of the enzyme in these cells relative to other cells of the caeaca. Scale bars: 350 µm (A), 250 µm (B), 200 µm (C).