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Fig. 4. Hanes–Woolf plots (concentration of amiloride, cAmi, versus ratio of cAmi to the induced change in Isc as a percentage of the control current, EAmi) of the influence of external amiloride on the currents across split gill lamellae at a clamp voltage of 50 mV (circles; mean 100 % control current=3635±475 µA cm–2) and on the currents across isolated cuticles at clamp voltages of 50 mV (squares; mean 100 % Icut=28 530±4889 µA cm–2) or 10 mV (triangles; mean 100 % Icut=4782±910 µA cm–2). Icut, cuticular current. The lines correspond to linear regressions (r2>0.99 in all cases) over the entire amiloride concentration range used (1.25–200 µmol l–1 for split gill lamellae and 0.5–100 µmol l–1 for isolated cuticles). Values are means ± S.E.M. (N=5 in all cases).





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