Fig. 8. ANP immunolocalisation in the ventricle of isolated amphibian heart perfused under various hyperosmotic conditions. Hearts were perfused under normal conditions (C) or subjected to various forms of hyperosmotic stress such as 0.5 mol l–1 sorbitol for 15 min in the absence (S) or presence of 1 µmol l–1 SB203580 (S+SB), 206 mmol l–1 NaCl for 2 min (NaCl) and 16 mmol l–1 KCl for 30 s (KCl), respectively. (A) Paraffin- or (B) cryo-sections were incubated with an antibody specific for human ANP (1–28) (1:500 dilution) and counterstained with Haematoxylin. In sections incubated only with secondary antibody (negative control, NC) no immunoreactivity was detected. The figure shows representative photographs from three independent experiments performed with similar results. Immunoreaction deposits are visualised with Fast Red chromogen. Bar, 20 µm.
