Fig. 6. p38-MAPK activity in response to hyperosmolarity (NaCl 206 mmol l–1 for 2 min) and hyperthermia (42°C for 30 s) in the perfused amphibian heart. (A) The kinase activity was assayed by the in-gel kinase method in extracts (200 µg of protein) from control hearts (C), hearts perfused with hypertonic medium (H) or perfused at increased temperature (T), as described in the Materials and Methods. A typical autoradiogram is shown, representative of at least three independent experiments. The molecular mass marker (kDa) is shown to the right. (B) Densitometric analysis of phospho-p38-MAPK bands by laser scanning. Values are means ± S.E.M. for three independent experiments performed with similar results. Asterisks indicate values significantly different from control values; *P<0.001, **P<0.01.
