Fig. 1. Time course of p38-MAPK phosphorylation in the amphibian heart, in response to reperfusion after sorbitol treatment. (Ai) Phospho-p38-MAPK was detected in extracts (100 µg of protein) from control hearts (C), hearts perfused with 0.5 mol l–1 sorbitol for 15 min (S), or hearts reperfused for the indicated times with normal bicarbonate-buffered saline following a 15 min perfusion with 0.5 mol l–1 sorbitol. Molecular mass markers (kDa) are shown to the right. (Aii) Identical samples immunoblotted for total p38-MAPK levels, as a control for loading. (B) Densitometric analysis of phospho-p38-MAPK bands by laser scanning. Results are means ± S.E.M. for three independent experiments performed with similar findings. Re, reperfusion. The western blots are representative of three independent experiments. *Significantly different from control value (P<0.001).
