Fig. 3. Effect of Ruthenium Red on 1 µmol l^–1 Phen Green fluorescence quenching. The activated form (which does not have to enter mitochondria and be activated by enzymes) of the dye was used in buffer containing the following concentrations of Ruthenium Red (RR): 0, 250, 500, 1000, 2500 and 5000 nmol l^–1. Fluorescence quenching (F) was followed over 120 s after the addition of Ruthenium Red. Changes in initial fluorescence are presented in figure. Values are means ± S.E.M., N=3. From this figure, only 5 % of the initial signal was lost at a Ruthenium Red concentration of 500 nmol l^–1, but 50 % was abolished when the inhibitor concentration was raised to 5000 nmol l^–1. The regression equation is F=543.6–0.057[RR], r²=0.99, P=0.001.

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