Fig. 3. Differential expression of hsp70 genes in larval gut as revealed by RNA:RNA in situ hybridization (RISH) using the 87A3'UTR (A,C,F), the 87C3'UTR (B,D,G) or the cDNA (E,H) riboprobe after 40 min of heat shock (HS) (A,B) or after 2 h (C,D,E) or 4 h (F,G H) of recovery (rec) from a 40 min heat shock. Insets in A and B show cells from the gut region at a higher magnification: inset 1 corresponds to cells from the middle part of the midgut (the two 3'UTR probes give a similar perinuclear signal in the cytoplasm); inset 2 shows a cell from the distal part of the midgut with strong 87A3'UTR hybridization; inset 3 is a cell from a similar position to that in inset 2 showing weak hybridization with the 87C3'UTR riboprobe. Scale bars (which apply to all images in a given row, except the insets), 200 µm.
