Fig. 1. Effect of the chronic presence of insulin and gliclazide on tyrosine phosphorylation of insulin receptor ß (IR-ß) in C2C12 myotubes. Gliclazide (2 mmol l^-1) was added during the last day of differentiation for 24 h to C2C12 cells differentiated in the absence (MF) or in the chronic presence (MFI) of insulin. The cells were then stimulated with 100 nmol l^-1 insulin for 5 min and lysed. Cell lysate was immunoprecipitated (IP) with antibodies against IR-ß and western immunoblotted (IB) with anti-phosphotyrosine (pTyr) antibody (A). The blots were stripped and reprobed with IR-ß (B). Experiments were repeated three times and representative blots are shown. Phosphorylation levels of IR (C) were quantified by densitometry and expressed relative to MF (control) samples. Error bars represent the S.E.M. of three independent experiments (^*P<0.05).

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