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Fig. 5. Identification of the active compound in the Nigella arvensis (NA) extract. (A) Fast protein liquid chromatography (FPLC) profile of the NA extract. The elution position of fraction 108, showing activity, is indicated by an arrow. (B) Further separation of fraction 108 by reverse-phase high-pressure liquid chromatography (RP-HPLC). The activity was demonstrated in a 215 nm peak eluting at 20% acetonitrile (fraction 12, indicated by an asterisk). The linear elution gradient is also shown.





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