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Fig. 3. Effects of the Nigella arvensis (NA) extract on kinetic parameters kob, kbo and KB of the interaction of 6-chloro-3,5-diaminopyrazine-2-carboxamide (CDPC) with the Na+ channel. (A) Effect of stepwise application of apical CDPC concentrations (ranging from 10 µmol l-1 to 100 µmol l-1) on short-circuit current (Isc) and transepithelial conductance (GT) of A6 cells before and after basolateral (Bl) stimulation with 250 µl l-1 NA extract (N=6). 50 µmol l-1 amiloride (Ami) was added after the highest dose of CDPC at the end of the experiment. Solutions were as in Fig. 1. (B) 2{pi}fc data at different doses of apical CDPC ([CDPC]Ap). The slope and intercept of the linear regressions for the control (2{pi}fc=7.66[CDPC]Ap+259.29) and in the presence of NA extract (2{pi}fc=7.64 [CDPC]Ap+281.51) indicate the ON and OFF rates, respectively, of the interaction between CDPC and the Na+ channel.





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