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Fig. 3. (A) A frequency histogram for the {lambda}max of all long wavelength cone scans, obtained from both aquarium-reared and wild-caught fish, calculated using the running average (`over-the-top' OTT) {lambda}max, but only presented if bandwidth data was also available from the individual scan. These data were used to calculate the ratio of bandwidth:OTT {lambda}max shown in (B). (B) Plot of OTT {lambda}max versus bandwidth:OTT {lambda}max, calculated for long-wavelength-sensitive visual pigment records, from black bream of different stages of development (diamonds). The lines labelled `A1-boundary' and `A2-boundary' model the bandwidth ratios of pure rhodopsin and porphyropsin pigments, respectively. The lines labelled `P5201 to P5751' and `P5201 to P5752' show the modelled bandwidth ratios of visual pigment mixtures formed firstly by two rhodopsins, and secondly by a hypothetical rhodopsin and porphyropsin. The best-fitting, two-peaked, curve (labelled `P5201 to P5501 to P5751') is provided by the model involving the mixture of these rhodopsins in sequentially expressed pairs





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