Fig. 3. A GABA-positive nonspiking interneuron in the metathoracic ganglion whose morphology can be directly related to previous descriptions; ventral view. (A) The cell body of the interneuron (arrow) appears yellow when the Lucifer-Yellow-fluorescence (green) and the Cy3/GABA-immunofluorescence (red) are merged, indicating colocalization of the dyes. Combined from three confocal planes each separated by 5.4 µm. (B) Morphology of the interneuron as revealed by the Lucifer Yellow dye (reconstructed from 41 confocal planes each separated by 5.4 µm). The primary neurite crosses the midline and then forms an extensive array of fine branches in neuropil close to the contralateral edge of the ganglion. A tracheole anterior to the cell body is strongly autofluorescent. Scale bars, 100 µm.

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