Fig. 4. Distribution of phospholipids and fatty acids in neonate and embryonic larvae. (A) BODIPY-labeled phosphatidylcholine analog (BODIPY-PC). (B) Location of phosphatidylcholine analog (BODIPY-PC) in newly hatched A. gambiae larvae. (C) Location of phosphatidylethanolamine analog (Texas Red-PE) in the same larvae. (D) Phase-contrast image of the larvae shown in B and C. Scale bar, 200µm. (E) Phase-contrast image of approximately 30-h-old embryonic A. gambiae larvae. Scale bar, 150µm. (F) Fluorescent image of the same larvae in E, showing the distribution of BODIPY-labeled maternal fatty acid (BODIPY-FA). (G) Image of the same embryonic larvae showing the distribution of the Texas Red-labeled maternal phosphatidylethanolamine (Texas Red-PE). (H) Diagram of a neonate A. gambiae larvae depicting the major accumulation sites of the maternal phospholipids (red) and fatty acids (green).

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