Fig. 1. Uptake and distribution of lipophorin, fatty acid and phosphatidylethanolamine by the ovary and oocyte of the mosquito Anopheles gambiae strain G3. (A) Uptake of ³²P-labeled lipophorin by different stages (Christophers, 1911) of the developing ovary. (B) Localization of FITC-labeled maternal lipophorin in developing oocytes. Scale bar, 25 µm. (C) Phosphatidylethanolamine analog labeled with Texas Red (Texas Red-PE, excitation 582 nm/emission 601 nm) on the polar head. (D) Fatty acid analog labeled with BODIPY (BODIPY-FA, excitation 505 nm/emission 515 nm) on the acyl chain. (E) Localization of the fatty acid (BODIPY-FA) in A. gambiae oocytes. Scale bar, 15 µm. (F) Localization of phospholipid (Texas Red-PE) in the same oocyte as in E. (G) Merged panels E and F demonstrate that fatty acids and phosphatidylethanolamine colocalized in the same vesicles.

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