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Fig. 4. (A) The contractile vacuole (CV) potential (filled squares), and the rate
of fluid segregation in the CV complex (RCVC; open
circles) of Paramecium multimicronucleatum as a function of the time
of re-exposure of the cells to their original hypotonic adaptation solution (4
mosmoll-1; abscissa) after they had received a prior exposure to a
hypertonic solution (124 mosmoll-1) for 30 min. Values are means
± S.E.M. (N=5-9). (B) DS-1 labelling of the decorated
spongiome, visualized in immunological fluorescence images. The number at the
top of each picture corresponds to the time (min) when the picture was taken
following re-exposure of the cell to the hypotonic solution. The control cell
was adapted to the hypotonic solution. Scale bar, 20 µm.