Fig. 5. The effect of thermal acclimation on the temperature sensitivity of HSF1 activation in liver tissue from G. mirabilis. Fish were collected in June, 2001 and either killed immediately (time 0), or acclimated for 5 weeks at 13°C, 21°C or 28°C. HSF1 activation levels were determined via electrophoretic mobility-shift assay. HSF1 activation levels are means ± S.E.M. (N=5). There was a significant effect of both heat shock temperature (P=0.011) and acclimation temperature (P<0.001) on HSF1 activation (ANOVA). Analyses of heat shock temperature effects within the acclimation groups are shown in Table 2. Analyses of the effect of acclimation group at a given heat shock temperature are listed in Table 3. ^*Within acclimation groups, heat shock temperature at which the mean HSF1 activity differed significantly (P<0.05; pairwise t-test) from that at the lowest heat shock temperature.

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