Fig. 2. Effect of NO donors on the embryonic rotation behavior. Application of SNAP (A) or SNP (B) at various concentrations resulted in a significant increase in the rate of embryonic rotation. In both cases, the most effective dose produced a twofold increase in rotation rate, in contrast to the fourfold increase produced by 100 µm serotonin (5-HT). (A) N=252 embryos; (B) N=147 embryos. (C) SNAP (10 µmol l^-1) and SNP (25 µmol l^-1) solutions that were pre-exposed to light for 24 h at room temperature (x-SNAP; x-SNP) had no effect on rotation rate. The NO-donating capability of SNAP and SNP are exhausted by this treatment, leaving only stable NO by-products. N=68 embryos. Asterisks represent statistically significant differences compared to the control (artificial pondwater, APW) treatment (P<0.05).

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