Fig. 4. Fluorescent dye used for detection of buffer solution leaks in microparabiotic chambers. 30 µl of buffer was delivered to the internal (left) side of the oothecal tissue, and 30 µl of buffer containing a radiolabeled material and fluorescein (50 µg ml^-1) was delivered to the external (right) side of the oothecal tissue prior to incubation. After various incubation times (0.5 h, 2 h, 6 h or 24 h), the microparabiotic chamber was viewed under ultraviolet light (256 nm) to detect leaks. (A) No detectable leakage. (B) Leak detected. Sample replication not used. Scale bar, 1 cm.

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