Fig. 4. Buffering capacity of KT semen and seminal plasma. (A) Representative titration of semen (filled squares) and seminal plasma (open squares) with HCl at 10°C. (B) Buffering capacity of semen (filled squares) and seminal plasma (open squares) as a function of pH. Each value shown was calculated as the added HCl (in µmol HCl ml^-1 semen or seminal fluid) divided by the resulting change in pH plotted against the median pH of each addition. Data represent 12-23 measurements from each of 6 males; curve represents the fourth order polynomial equation generated using all data points. (C) Buffering capacity of semen and seminal (Sem.) plasma determined by linear regression analysis of titration curves over the pH range 7.5-8.5 and 6.0-7.0; N=6. Buffering capacities of semen and seminal plasma were not different over either pH range. (Comparable data were obtained for UCD samples, N=4; data not shown.)

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