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Fig. 2. Immunoblots (western blots) of caterpillar and moth tissue samples probed
with the N- and C-terminal-specific TrnEAAT1 antibodies. (A) Tissue extracts
probed with the antibody to the C-terminal peptide; (B) extracts probed with
antibody to the N-terminal peptide. Lanes: 1, moth head membrane fraction; 2,
caterpillar skeletal muscle membrane fraction; 3, moth head water-soluble
fraction; 4, caterpillar skeletal muscle water-soluble fraction. The
transporter bands are detected in the membrane fractions as in
Fig. 1, and the positions of
marker proteins (kDa) are shown.